Nucleotide sequences for Oncorhynchus kisutch

VTG

BLAST

PRIMERS

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Primer pair 1

	Sequence (5'->3')	Template strand	Length	Start	Stop	Tm	GC%	Self complementarity	Self 3' complementarity
Forward primer	TGCTCCTGGGTGGTCTGCCA	Plus	20	419	438	59.75	65.00	3.00	3.00
Reverse primer	CGTTGGGCCACACACCACTGT	Minus	21	659	639	59.78	61.90	5.00	3.00
Product length	241

Primer pair 2

	Sequence (5'->3')	Template strand	Length	Start	Stop	Tm	GC%	Self complementarity	Self 3' complementarity
Forward primer	AGGCACTGCTCCTGGGTGGT	Plus	20	413	432	59.75	65.00	5.00	1.00
Reverse primer	TCGTTGGGCCACACACCACTG	Minus	21	660	640	59.51	61.90	5.00	3.00
Product length	248

Primer pair 3

	Sequence (5'->3')	Template strand	Length	Start	Stop	Tm	GC%	Self complementarity	Self 3' complementarity
Forward primer	GCTGGAGCTCAGGGAGTGTGC	Plus	21	953	973	59.45	66.67	6.00	2.00
Reverse primer	AGCTCCCATCAGGGCCTCCC	Minus	20	1228	1209	59.67	70.00	5.00	3.00
Product length	276

Primer pair 4

	Sequence (5'->3')	Template strand	Length	Start	Stop	Tm	GC%	Self complementarity	Self 3' complementarity
Forward primer	TGCCAAGGCAGAACGCATCCA	Plus	21	1000	1020	59.31	57.14	6.00	0.00
Reverse primer	GTGGCAGCTCCCATCAGGGC	Minus	20	1233	1214	59.76	70.00	8.00	2.00
Product length	234

Primer pair 5

	Sequence (5'->3')	Template strand	Length	Start	Stop	Tm	GC%	Self complementarity	Self 3' complementarity
Forward primer	GGCACTGCTCCTGGGTGGTC	Plus	20	414	433	59.34	70.00	5.00	1.00
Reverse primer	TCGTTGGGCCACACACCACT	Minus	20	660	641	58.48	60.00	5.00	1.00
Product length	247

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